The PLEX-ID Vector-Borne BPN assay is a qualitative, non-diagnostic assay intended for the detection and identification of a broad range of vector-borne pathogens directly from whole blood, CSF, serum, plasma, skin biopsies, fleas, mosquitoes, or ticks, through nucleic acid amplification and subsequent mass spectrometry analysis. This assay is intended for use for companion and production animal health, public health surveillance, or scientific research.
*Magnetic beads and clean-up reagents are included with the T5000 assay but must be purchased separately for use with the PLEX-ID assay.
The PLEX-ID Vector-Borne BPN assay kit consists of:
- 10 Plates (120 assays)
- Protocol for Processing PLEX-ID Vector-Borne BPN Assay
- DNA Dilution Buffer, 8ml (1)
Positive, negative, or extraction controls are not provided.
There is no specific sample extraction protocol available for this assay, however, fluid handling scripts may be available for the PLEX-ID FH, based upon the assay plate configuration, volume, reagents and sample format required.
The PLEX-ID Vector-Borne BPN assay can detect and identify the following microbes associated with infections transmitted by ticks:
- Bacteria
- Francisella species such as F. tularensis and subspecies.
- Lyme disease Borrelia, such as B. burgdorferi, B. afzelii, B. valasiana, B. spielmanii, B. lusitaniae and B. garinii.
- Rickettsia pathogens like R. rickettsii, R. typhi, and other non-pathogenic Rickettsia.
- Various alphaproteobacteria; Ehrlichia spp., Anaplasma phagocyophilum, Bartonella spp.
- Protozoa
- Babesia protozoa such as B. microti, B. divergens, B. bovis, B. gibsoni, and B. canis.
- Filarial nematodes
- Canine heartworm: Dirofilaria immitis
