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Technical FAQs

What is the basis for the Ibis T5000 Biosensor System?
The Ibis T5000 Biosensor System takes standard polymerase chain reaction (PCR) products, which are produced using Ibis assay kits, cleans the amplicons, and measures their masses on a microTOF mass spectrometer. These masses are used to link the amplicons to specific organisms or markers.
Why is the Ibis T5000 Biosensor System technology a fundamentally different technology?
The Ibis T5000 Biosensor System was designed as a research tool to find bacteria, viruses, fungi, or potentially any other nucleic acid in your samples-without knowing beforehand exactly what it is looking for. Additionally, the Ibis T5000 Biosensor System gives more than just a yes/no answer. A sample "fingerprint" is obtained, and this allows in-depth analysis.
Why does the Ibis T5000 Biosensor System need a mass spectrometer?
The mass spectrometer takes the PCR amplicons and gets very precise masses for the forward and reverse strands. Using these masses, the IbisTrack analytical system is able to determine the amplicon base composition - the exact number of A's, G's, C's, and T's in that amplicon. The base composition is then used to look up the organism identification in Ibis' proprietary amplicon database.
Do I need to look at mass spectra to interpret results?
No. IbisTrack calculates everything for you and produces simple results in an easily understood format.
Can the Ibis T5000 Biosensor System determine the components of mixtures?
Yes, the Ibis T5000 Biosensor System was designed as a research tool to detect multiple types of organisms present in the same sample.
How many samples can the Ibis T5000 Biosensor System process in one day?
In a 24-hour period, approximately 1400 wells can be processed. For a typical eight-well assay, this translates to 180 samples.
What types of nucleic acid isolation may be used?

Any protocol that properly isolates nucleic acid into a clean sample without inhibitors may be used. For suggested protocols, please contact us.
Is the Ibis T5000 Biosensor System really used for forensics?
Yes. It is used by the Federal Bureau of Investigation (FBI) to do forensic analysis of mitochondrial DNA.

 

General FAQs

What is Ibis Biosciences?
Ibis Biosciences, Inc. is a wholly-owned subsidiary of Abbott Molecular, Inc. To learn more, please visit the Abbott Molecular page.
What are the site requirements for an Ibis T5000 Biosensor System?
Contact us to receive our site requirements guide.
Are products from Ibis Biosciences available outside of the United States?
Yes. Please contact us for details.
How can I learn more about the Ibis T5000 Biosensor System?
Feel free to review our many publications or attend one of our upcoming presentations. Links to these are being updated and will be available shortly. You may contact us anytime to ask questions, schedule a visit, or request ordering information.

 

 

 

The Ibis T5000 Biosensor System was designed as a research tool tofind bacteria, viruses, fungi, or potentially any other nucleic acid inyour samples-without knowing beforehand exactly what it is looking for.Additionally, the Ibis T5000 Biosensor System gives more than just ayes/no answer. A sample "fingerprint" is obtained, and this allowsin-depth analysis.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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